ELECTROPORATION-MEDIATED GENE TRANSFER TO PROTOPLASTS OF ASPARAGUS OFFICINALIS L.
نویسندگان
چکیده
منابع مشابه
Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts
In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form were evaluated. Transformation efficiency was assayed in vivo as...
متن کاملElectroporation-mediated gene transfer to the developing mouse inner ear.
The mammalian inner ear forms from a thickened patch of head ectoderm called the otic placode. The placodal ectoderm invaginates to form a cup whose edges cinch together to establish a fluid-filled sac called the otic vesicle or otocyst. The progenitor cells lining the otocyst lumen will give rise to sensory and non-sensory cells of the inner ear. These formative stages of inner ear development...
متن کاملElectroporation-mediated gene transfer system applied to cultured CNS neurons.
Electroporation is effective in transferring foreign genes into immature neurons in intact brain tissue. We utilized this approach to transfect genes into developing rodent hippocampi. Transfected hippocampi were subsequently dissociated and allowed to differentiate in culture. By optimizing developmental stage of the hippocampus, promoters to drive the marker cDNA, and culture conditions, neur...
متن کاملRoot Residue Amendment on Varietal Allelopathy and Autotoxicity of Replanted Asparagus (Asparagus officinalis L.)
This study evaluated the allelopathic potential and autotoxic interference with growth and nutrient uptake of two asparagus varieties; UC157 (U) and Gijnlim (G) from USA and Europe, respectively. The two varieties were cultivated in a continuous replanting system in different rotational patterns with soil amended by asparagus root residues, and unamended soil (control) under greenhouse conditio...
متن کاملJEC BB 01516 Membrane electroporation and direct gene transfer l
The direct transfer of genetic material into cells by electroporation can be described in physicochemical terms as an electroporation-resealing hysteresis. The hysteresis concept includes unidirectional state transitions of the membrane, coupled to electrodiffusive migration of DNA through cell wall structures and electroporated plasma membranes. Deeper insight into electroporation phenomena su...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: HortScience
سال: 1994
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.29.4.251g